| 產(chǎn)品編號 | bs-1170R |
| 英文名稱 | FOS B Rabbit pAb |
| 中文名稱 | FosB抗體 |
| 別 名 | FBJ murine osteosarcoma viral oncogene homolog B; FOS B; FOS-B; FBJ osteosarcoma oncogene B; fosB; G0/G1 switch regulatory protein 3; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOS 3; GOS B; GOSB; MGC42291; Oncogene FOS B; Oncogene FOSB; Protein fos B; Protein fosB; DKFZp686C0818; FOSB_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 染色質和核信號 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOS B: 191-290/338 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, Fos B, (fos-related antigen) Fra 1, and Fra 2. Also named as DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB. Function: FosB interacts with Jun proteins enhancing their DNA binding activity. Subunit: Heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P53539 Gene ID: 2354 Database links: Entrez Gene: 2354 Human Omim: 164772 Human SwissProt: P53539 Human Unigene: 590958 Human 轉錄調節(jié)因子（Transcriptin Regulators） FOS B屬于轉錄因子AP-1家族;FosB是一種轉錄因子，表達后能在腦內(nèi)維持數(shù)周甚至幾個月。長期藥物濫用能引起伏核內(nèi)FosB表達穩(wěn)定地增加，表明FosB的表達可能是藥物成癮的分子轉換機制，是當今藥物成癮研究中最受重視的轉錄因子。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOS B) Polyclonal Antibody, Unconjugated (bs-1170R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
