| 產(chǎn)品編號 | bsm-61924R |
| 英文名稱 | IQGAP1 Recombinant Rabbit mAb |
| 中文名稱 | 支架蛋白重組兔單抗 |
| 別 名 | Iqgap1; p195; SAR1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 189 kDa |
| 檢測分子量 | 180 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IQGAP1: 1-58/1657 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. SWISS: P46940 Gene ID: 8826 支架蛋白IQGAP1與許多重要的細胞活動緊密相關(guān)，如黏附和遷移。研究發(fā)現(xiàn)在損傷修復(fù)、組織發(fā)展、腫瘤轉(zhuǎn)移等過程中，IQGAP1的表達和功能有明顯改變。同時作為Rh.GTPase信號的主要效應(yīng)因子，IQGAP1能傳遞細胞外信號至黏附素復(fù)合體、細胞骨架以及細胞核。通過上述信號通路，IQ-GAP1協(xié)助細胞完成一系列重要生理病理活動。 |
| 產(chǎn)品圖片 |
Western blot analysis of HeLa cell lysate. Using IQGAP1 (bsm-61924R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
