| 產(chǎn)品編號(hào) | bsm-61830R |
| 英文名稱(chēng) | NEDD4 Recombinant Rabbit mAb |
| 中文名稱(chēng) | 神經(jīng)前體細(xì)胞發(fā)育下調(diào)蛋白4重組兔單抗 |
| 別 名 | Nedd4; PIG53; RPF1. |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 149 kDa |
| 檢測(cè)分子量 | 135 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NEDD4: 1271-1319 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. SWISS: P46934 Gene ID: 4734 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with NEDD4 monoclonal antibody, unconjugated (bsm-61830R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NEDD4) monoclonal Antibody, unconjugated (bsm-61830R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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