產(chǎn)品編號 | bsm-61766R |
英文名稱 | IMP3 Recombinant Rabbit mAb |
中文名稱 | 胰島素樣生長因子2mRNA結(jié)合蛋白3重組兔單抗 |
別 名 | CT98; hKOC; IGF2BP3; IMP 3; KOC1; VICKZ 3. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 64 kDa |
檢測分子量 | 69 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human IMP 3: 1-200 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. SWISS: O00425 Gene ID: 10643 IGF2BP3是一種可以與胰島素樣生長因子(IGFs)結(jié)合的分泌性蛋白,其通過IGFs調(diào)節(jié)細(xì)胞的生長、分化、凋亡、黏附和運(yùn)動(dòng),IGF2BP3與IGFs結(jié)合可以調(diào)節(jié)IGFs與IGF2型受體間的相互作用,改變其促有絲分裂效應(yīng)、凋亡抑制效應(yīng)和促細(xì)胞運(yùn)動(dòng)作用。 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with IMP3 monoclonal antibody, unconjugated (bsm-61766R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IMP3 Monoclonal Antibody, Unconjugated(bsm-61766R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|