產(chǎn)品編號 | bsm-61754R |
英文名稱 | Mammaglobin A Recombinant Rabbit mAb |
中文名稱 | 乳腺珠蛋白1重組兔單抗 |
別 名 | Mammaglobin 1; Mammaglobin-A; MGB1; Scgb2a2; SG2A2; UGB2. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | |
交叉反應(yīng) | Human (predicted: Rat) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 10 kDa |
檢測分子量 | 18 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human Mammaglobin A: 1-93 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
SCGB2A2 Belongs to the secretoglobin family. Lipophilin subfamily. 2 isoforms of the human protein are produced by alternative splicing. SWISS: Q13296 Gene ID: 4250 乳腺球蛋白是一個乳腺相關(guān)糖蛋白,它是一個分泌素家族(包括子宮球蛋白和lipophilin)成員的遠親。與其他分泌素家族成員不同,乳腺球蛋白的mRNA在乳腺組織中特異表達。在石蠟組織切片的檢測中,該抗體檢測乳腺癌組織中的乳腺珠蛋白敏感度高達80%。當和其他乳腺特異標記如GCDFP-15聯(lián)合應(yīng)用時,檢測乳腺珠蛋白敏感度高達84%。有資料表明檢測乳腺球蛋白,在鑒定原發(fā)癌是否發(fā)生轉(zhuǎn)移中起到至關(guān)重要的作用。 |
產(chǎn)品圖片 |
Western blot analysis of Human breast cancer lysate. Using Mammaglobin A (bsm-61754R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Mammaglobin A Monoclonal Antibody, Unconjugated(bsm-61754R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |