| 產(chǎn)品編號 | bsm-61741R |
| 英文名稱 | Endo G Recombinant Rabbit mAb |
| 中文名稱 | 核酸內(nèi)切酶G重組兔單抗 |
| 別 名 | EndoG; EndonucleaseG; Mitochondrial endonuclease G; NUCG_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10F16 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33 kDa |
| 檢測分子量 | 30 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Endo G: 250-297/297 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. SWISS: Q14249 Gene ID: 2021 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Endo G monoclonal antibody, unconjugated (bsm-61741R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Western blot analysis of HepG2 cell lysate. Using Endo G (bsm-61741R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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