| 產品編號 | bsm-61720R |
| 英文名稱 | IKK epsilon Recombinant Rabbit mAb |
| 中文名稱 | 核因子κB抑制蛋白E重組兔單抗 |
| 別 名 | IKBKE; IKK-E; IKK-i; IKKE; IKKI; IKKi/IKKe. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | 80 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IKK epsilon: 32-65 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death. SWISS: Q14164 Gene ID: 9641 I kappa B kinase epsilon(IKKe)也是絲氨酸/蘇氨酸蛋白激酶家族.可以磷酸化IkB，從而激活NF-kB通路，促進NF-kB核移位,并最終降解NF-kB的抑制劑. IKKe可表達在很多組織細胞中;胸腺、胰腺、脾、胎盤、外周血白細胞，也可少量表達在肺、腎、前列腺癌、卵巢、結腸。但最多表達在乳腺腫瘤上，IKBKE在人類乳腺癌中明顯高表達，這種新的乳腺癌易感基因在腫瘤的形成和發(fā)展中起著及其關鍵的作用。IKBKE蛋白不同于乳腺癌易感基因BRCA1和BRCA2，是先天具有的。帶有這些遺傳變異 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with IKK epsilon monoclonal antibody, unconjugated (bsm-61720R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
