| 產(chǎn)品編號 | bsm-61719R |
| 英文名稱 | Heparanase Recombinant Rabbit mAb |
| 中文名稱 | 乙酰肝素酶重組兔單抗 |
| 別 名 | HEP; Heparanase; Heparanase1; Hpa 1; HPA; Hpa1 HPR1; HPSE1; HSE1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61 kDa |
| 檢測分子量 | 70 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Heparanase: 1-60/543 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. SWISS: Q9Y251 Gene ID: 10855 乙酰肝素酶(heparanase)在血管形成、炎性反應、組織生長發(fā)育及腫瘤轉(zhuǎn)移的過程中起著重要作用。在正常情況下,乙酰肝素酶的分泌和活性必須受到嚴格的調(diào)控,以免其表達對細胞外基質(zhì)錯誤切割造成組織損傷而發(fā)生各種病理改變。 |
| 產(chǎn)品圖片 |
Western blot analysis of K562 cell lysate. Using Heparanase (bsm-61719R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
