產(chǎn)品編號 | bsm-61689R |
英文名稱 | ZEB1 Recombinant Rabbit mAb |
中文名稱 | 負調(diào)控因子白細胞介素2重組兔單抗 |
別 名 | BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 4C1 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:200-1:1000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 124 kDa |
檢測分子量 | 200 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human ZEB1: 1-174 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. SWISS: P37275 Gene ID: 6935 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ZEB1 monoclonal antibody, unconjugated (bsm-61689R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZEB1 Monoclonal Antibody, Unconjugated(bsm-61689R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZEB1 Monoclonal Antibody, Unconjugated(bsm-61689R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZEB1 Monoclonal Antibody, Unconjugated(bsm-61689R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZEB1 Monoclonal Antibody, Unconjugated(bsm-61689R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|