| 產(chǎn)品編號 | bsm-61622R |
| 英文名稱 | Phospho-CRK (Tyr221) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化CRK重組兔單抗 |
| 別 名 | Adapter molecule crk; avian sarcoma virus CT10(v-crk) oncogene homolog; CRK; CRKII; Proto-oncogene c-Crk. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | 42 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CRK around the phosphorylation site of Y221: GP-pY-AQ 216-226/304 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. SWISS: Q96HJ0 Gene ID: 1398 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) K562 cell lysate treated with AP; (2) K562 cell lysate. Using Phospho-CRK (Tyr221) (bsm-61622R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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