產(chǎn)品編號 | bsm-61601R |
英文名稱 | Emerin Recombinant Rabbit mAb |
中文名稱 | Emerin蛋白重組兔單抗 |
別 名 | EDMD; Emd; Emerin; Emery Dreifuss muscular dystrophy; STA. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 13F1 |
交叉反應(yīng) | Human,Mouse (predicted: Rat) |
產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-1:500,IHC-F=1:100-1:500,IF=1:100-1:500,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 29 kDa |
檢測分子量 | 29 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human Emerin: 200-254/254 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Emerin may regulate gene expression through binding to other transcriptional regulators. Emerin binds to β-catenin and inhibits its nuclear accumulation. Recent studies demonstrate that Emerin is required for HIV-1 infectivity. SWISS: P50402 Gene ID: 2010 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Emerin monoclonal antibody, unconjugated (bsm-61601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Emerin Monoclonal Antibody, Unconjugated(bsm-61601R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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