| 產(chǎn)品編號 | bsm-61574R |
| 英文名稱 | Calpain 2 Recombinant Rabbit mAb |
| 中文名稱 | 鈣激活的中性蛋白酶2重組兔單抗 |
| 別 名 | CALP80; Calpain M type; Calpain-2 large subunit; Calpain2; CANP2; CANPL2; CANPml; Capa2; CAPN2; M calpain; mCANP; Millimolar calpain. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | 80 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CAPN2: 657-700/700 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. SWISS: P17655 Gene ID: 824 鈣蛋白酶2(calpain2)也是一族鈣離子依賴性的、水解半胱氨酸的蛋白酶,廣泛存在于全身各臟器組織中。鈣蛋白酶參與不同細胞過程,包括細胞骨架和細胞膜附著的構(gòu)型重建、不同的信號轉(zhuǎn)導途徑和細胞凋亡等. |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Calpain 2 monoclonal antibody, unconjugated (bsm-61574R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
