| 產(chǎn)品編號 | bsm-61566R |
| 英文名稱 | CYP3A4 Recombinant Rabbit mAb |
| 中文名稱 | 細胞色素P450 3A4重組兔單抗 |
| 別 名 | CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1F9 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-1:500,IHC-F=1:100-1:500,IF=1:100-1:500,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | 57 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Cytochrome P450 3A4: 450-503/503 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. SWISS: P08684 Gene ID: 1576 細胞色素3A4(CYP3A4)是細胞色素P450 超家族成員之一, 主要存在于肝臟中和小腸中。它是人類藥物代謝酶中的最重要的酶, CYP3A4與外源性致癌物、抗癌藥物和內(nèi)源性類固醇激素、維生素、脂肪酸的代謝轉(zhuǎn)化密切相關(guān)。 |
| 產(chǎn)品圖片 |
Western blot analysis of Human fetal liver lysate. Using CYP3A4 (bsm-61566R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CYP3A4 Monoclonal Antibody, Unconjugated(bsm-61566R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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