產(chǎn)品編號(hào) | bsm-61540R |
英文名稱(chēng) | BAG3 Recombinant Rabbit mAb |
中文名稱(chēng) | Bcl2抑凋亡蛋白Bag3重組兔單抗 |
別 名 | BAG 3; BAG family molecular chaperone regulator 3; Bag3; Bcl 2 binding protein; Bcl-2-binding protein Bis; BIS; CAIR 1; Docking protein CAIR 1; MFM6. |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Recombinant |
克 隆 號(hào) | 15A17 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 62 kDa |
檢測(cè)分子量 | 80 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human BAG3: 60-130/575 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. SWISS: O95817 Gene ID: 9531 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with BAG3 monoclonal antibody, unconjugated (bsm-61540R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BAG3 Monoclonal Antibody, Unconjugated(bsm-61540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BAG3 Monoclonal Antibody, Unconjugated(bsm-61540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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