| 產品編號 | bsm-61489R |
| 英文名稱 | Involucrin Recombinant Rabbit mAb |
| 中文名稱 | 囊包蛋白/內披蛋白重組兔單抗 |
| 別 名 | Involucrin; IVL. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10A18 |
| 交叉反應 | Human (predicted: Rat) |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,Flow-Cyt=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測分子量 | 120 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Involucrin: 100-200 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. SWISS: P07476 Gene ID: 3713 套膜蛋白又稱內披蛋白或囊包蛋白，與毛透明蛋白共同構成角質細胞包膜支架的蛋白質。在角質細胞分化時，首先出現在表皮棘層的上層細胞中的標志性蛋白質，該蛋白在角質細胞和其他復層扁平上皮合成的蛋白。最初存在于細胞質中，然后通過轉谷氨酰胺酶的作用，轉變?yōu)榻宦?lián)的膜蛋白。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Involucrin monoclonal antibody, unconjugated (bsm-61489R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
