| 產(chǎn)品編號(hào) | bsm-61440R |
| 英文名稱(chēng) | BNIP3 Recombinant Rabbit mAb |
| 中文名稱(chēng) | 促凋亡調(diào)節(jié)蛋白BNIP3重組兔單抗 |
| 別 名 | BNIP3; NIP3. |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測(cè)分子量 | 30,50; |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human BNIP3: 100-150 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. SWISS: Q12983 Gene ID: 664 BNIP3蛋白在缺氧條件下可誘導(dǎo)細(xì)胞發(fā)生細(xì)胞凋亡。BNIP3促細(xì)胞凋亡線粒體途徑根據(jù)有無(wú)caspase的參與而具有不同的作用機(jī)制，也是研究的熱點(diǎn)。BNIP3還可誘導(dǎo)細(xì)胞自噬。BNIP3全稱(chēng)為Bcl2/腺病毒E1B19kD相互作用蛋白3。(Bcl2/adenovirusE1B19kDainteractingprotein3，BNIP3)，是新近發(fā)現(xiàn)的BH3only亞家族成員之一，也是低氧敏感的凋亡基因。在化學(xué)性低氧誘導(dǎo)細(xì)胞凋亡的過(guò)程中，ATP是BNIP3導(dǎo)致細(xì)胞凋亡的關(guān)鍵性因素之一。單獨(dú)ATP下降不誘導(dǎo)細(xì)胞 |
| 產(chǎn)品圖片 |
Hela (H) cells were treated with or without cobalt chloride (100 μM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with BNIP3 monoclonal antibody, unconjugated (bsm-61440R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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