| 產(chǎn)品編號 | bsm-61433R |
| 英文名稱 | Cathepsin L/V/K/H Recombinant Rabbit mAb |
| 中文名稱 | 組織蛋白酶L/V/K/H重組兔單抗 |
| 別 名 | Cathepsin; CTSL; CTSV; CTSK; CTSH. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37,38 kDa |
| 檢測分子量 | 38 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Cathepsin L/V/K/H: 110-135 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. SWISS: P07711 Gene ID: 1514 合成與降解（Synthesis and Degradation） 組織蛋白酶L(cathepsin L CL)屬于木瓜蛋白酶家族中的半胱氨酸蛋白水解酶，以酶原的形式貯存于溶酶體中. 參與許多特殊的生理過程，如激素原的激活、抗原呈遞、組織器官的發(fā)育等。 在病理狀態(tài)下，因各種原因所致的細胞損傷(如病原微生物、炎癥因子、氧化應激等)可降解細胞成分或細胞間質(zhì)基質(zhì)成分，包括層粘素、IV型膠原纖維及纖維連接素等，與人類許多疾病如腫瘤的浸潤與轉(zhuǎn)移、關節(jié)炎、骨質(zhì)疏松、阿爾茨海姆病、多發(fā)性硬化癥及其他慢性炎癥性疾病有關. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Invasive urothelial carcinoma of the bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cathepsin L/V/K/H Monoclonal Antibody, Unconjugated (bsm-61433R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
