| 產(chǎn)品編號 | bsm-61420R |
| 英文名稱 | TAC1 Recombinant Rabbit mAb |
| 中文名稱 | 神經(jīng)激肽A重組兔單抗 |
| 別 名 | Protachykinin-1; PPT; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; NPK; TAC1; NKA, NKNA, TAC2; Tachykinin 1; tachykinin 2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 17A9 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | 15 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TAC1: 50-100/129 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. SWISS: P20366 Gene ID: 6863 P物質(zhì)（Substance P）是腦組織及消化道中發(fā)現(xiàn)的一種神經(jīng)遞質(zhì)肽。由130個氨基酸殘基組成，胃腸道中肌肉收縮的有力促進劑，亦為強血管擴張劑。 SP是一種腦腸肽，亦是最早發(fā)現(xiàn)的一種神經(jīng)肽，屬于速激肽家族成員，它廣泛分布于中樞及外周神經(jīng)系統(tǒng)，作為一種神經(jīng)遞質(zhì)或調(diào)質(zhì)參與疼痛、免疫、心血管等多種生理功能的調(diào)節(jié)。SP與其它的遞質(zhì)或調(diào)質(zhì)類似，能作用于多種類型的受體，速激肽家族有NK1、NK2、NK3三種受體，都能與SP結合，其中NK1與SP的結合能力最強而被稱為SP受體。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TAC1 Monoclonal Antibody, Unconjugated (bsm-61420R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TAC1 Monoclonal Antibody, Unconjugated (bsm-61420R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TAC1 Monoclonal Antibody, Unconjugated (bsm-61420R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
