| 產(chǎn)品編號 | bsm-61287R |
| 英文名稱 | ILK Recombinant Rabbit mAb |
| 中文名稱 | 整合素連接激酶ILK重組兔單抗 |
| 別 名 | ILK; DKFZp686F1765; P59; ILK1; Integrin linked protein kinas. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10G2 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | 51 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ILK: 401-452/452 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. SWISS: Q13418 Gene ID: 3611 ILK 是一種新發(fā)現(xiàn)的Ser/Thr蛋白激酶。ILK能夠通過與整合素β1亞單位的結(jié)合介導細胞與胞外基質(zhì)的連接，以依賴于PI3K的方式激活，并通過磷酸化下游底物PKB/AKT,GSK3等胞外信號的一項下游傳遞，對細胞的生長，分化，遷移等進行調(diào)控。由于ILK在胞內(nèi)外信號傳導中起著重要的作用。并且抑制ILK的活性能夠?qū)е录毎芷诘耐图毎绦蛐运劳龅膯?，使其成為腫瘤治療和腫瘤藥物的理想靶位點。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ILK monoclonal antibody, unconjugated (bsm-61287R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
