| 產(chǎn)品編號 | bsm-61253R |
| 英文名稱 | Radixin Recombinant Rabbit mAb |
| 中文名稱 | 根蛋白重組兔單抗 |
| 別 名 | RDX; Radixin; Protein roadkill; HIB; ESP10; DFNB24; RADI. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-1:5000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69 kDa |
| 檢測分子量 | 80 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Radixin: 350-430/583 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane. SWISS: P35241 Gene ID: 5962 根蛋白是一種細胞骨架蛋白，在連接肌動蛋白于質(zhì)膜中可能有重要作用。研究表明，根蛋白對于結(jié)合膽紅素通過其Mrp2在BCMs中定位支持的分泌是需要的。埃茲蛋白-根蛋白-膜突蛋白家族蛋白是亞膜皮質(zhì)，特別是上皮細胞中普遍存在的成分。其可能參與將肌動蛋白絲的頂端錨定于胞膜。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Radixin monoclonal antibody, unconjugated (bsm-61253R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
