| 產品編號 | bsm-61175R |
| 英文名稱 | DARPP32 Recombinant Rabbit mAb |
| 中文名稱 | 多巴胺/cAMP調節(jié)神經磷蛋白重組兔單抗 |
| 別 名 | DARPP32; PPP1R1B; PPR1B; Protein phosphatase 1 regulatory subunit 1B. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | 32 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DARPP 32 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. SWISS: Q9UD71 Gene ID: 84152 ????DARPP-32通過自身不同位點的磷酸化對蛋白質磷酸化和去磷酸化過程發(fā)揮著雙向調控的作用.DARRP-32在腦內存在于接受多巴胺能投射的神經元中,其磷酸化過程受多種神經遞質的調控,在信號轉導途徑中處于中心位置,整合多種胞內信號轉導,調節(jié)神經元的電學和化學性質以及動物的生理行為反應,參與多種生理功能和病理過程,包括藥物成癮、抑郁癥、精神分裂癥等.此外,DARPP-32在非神經系統(tǒng)的功能也逐漸被人們所認識. ????多巴胺和cAMP調節(jié)的磷蛋白(dopamine and adenosine 3 |
| 產品圖片 |
Western blot analysis of Rat brain lysate. Using DARPP32 (bsm-61175R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
